NMR spectroscopy based metabolic profiling of urine and serum for investigation of physiological perturbations during radiation sickness |
| |
Authors: | Ahmad Raza Khan Poonam Rana Ritu Tyagi Indracanti Prem Kumar M Memita Devi Salim Javed Rajendra P Tripathi Subash Khushu |
| |
Affiliation: | (1) NMR Research Centre, Institute of Nuclear Medicine and Allied Sciences (INMAS), DRDO, Lucknow Road, Timarpur, Delhi, India;(2) Division of Radiation Biosciences, Institute of Nuclear Medicine and Allied Sciences (INMAS), Delhi, India;(3) Department of Biochemistry, Jamia Hamdard, New Delhi, India; |
| |
Abstract: | Radiation accidents are rare events that induce radiation syndrome, a complex pathology which is difficult to treat. In medical
management of radiation victims, life threatening damage to different physiological systems should be taken into consideration.
The present study was proposed to identify metabolic and physiological perturbations in biofluids of mice during different
phases of radiation sickness using 1H nuclear magnetic resonance (1H NMR) spectroscopy and pattern recognition (PR) technique. The 1H NMR spectra of the biofluids collected from mice irradiated with 5 Gray (Gy) at different time points during radiation sickness
were analysed visually and by principal components analysis. Urine and serum spectral profile clearly showed altered metabolic
profiles during different phases of radiation sickness. Increased concentration of urine metabolites viz. citrate, α ketoglutarate,
succinate, hippurate, and trimethylamine during prodromal and clinical manifestation phase of radiation sickness shows altered
gut microflora and energy metabolism. On the other hand, serum nuclear magnetic resonance (NMR) spectra reflected changes
associated with lipid, energy and membrane metabolism during radiation sickness. The metabonomic time trajectory based on
PR analysis of 1H NMR spectra of urine illustrates clear separation of irradiated mice group at different time points from pre dose. The difference
in NMR spectral profiles depicts the pathophysiological changes and metabolic disturbances observed during different phases
of radiation sickness, that in turn, demonstrate involvement of multiple organ dysfunction. This could further be useful in
development of multiparametric approach for better evaluation of radiation damage as well as for medical management during
radiation sickness. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|