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Southern blotting analyses of strains ofCampylobacter fetus using the conserved region ofsapA |
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| Authors: | Masaki Fujita Tatsuya Morooka Shuji Fujimoto Tetsuhiro Moriya Kazunobu Amako |
| Institution: | (1) Department of Bacteriology, Faculty of Medicine, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812, Japan Tel. +81-92-641-1151 (Ext. 3406); Fax +81-92-633-4257 e-mail fujita@kokyu.med.kyushu-u.ac.jp, JP;(2) Department of Pediatrics, Chikushi Hospital, Fukuoka University, Fukuoka, Japan, JP |
| Abstract: | Chromosomal DNA of 27 strains of Campylobacter fetus was analyzed by Southern blotting with a probe of the conserved region of sapA. The probe hybridized with 23 strains that produced type A lipopolysaccharide. These strains had more than six sapA homologs. In Southern blots of SalI-digested chromosomal DNA separated by pulsed-field gel electrophoresis, one fragment from 19 strains and two fragments from 4 strains hybridized. These data indicate that multiple sapA homologs are localized to a limited region on the chromosomal DNA of C. fetus and thus suggest the possibility of developing a typing system using this method. Received: 28 June 1995 / Accepted: 19 September 1995 |
| Keywords: | Campylobacter fetus sapA Conserved region S-layer Pulsed field gel electrophoresis Southern blot Strain typing |
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