Influenza virus hemagglutinin expression is polarized in cells infected with recombinant SV40 viruses carrying cloned hemagglutinin DNA |
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| Authors: | M G Roth R W Compans L Giusti A R Davis D P Nayak M J Gething J Sambrook |
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| Affiliation: | 1. Department of Microbiology University of Alabama in Birmingham Birmingham, Alabama 35294 USA;2. University of California, Los Angeles Jonsson Comprehensive Cancer Center and Department of Microbiology and Immunology University of California, Los Angeles, School of Medicine, Los Angeles, California 90024 USA;3. Cold Spring Harbor Laboratory Cold Spring Harbor, New York 11724 USA |
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| Abstract: | Primary cell cultures of African Green monkey kidney (AGMK) contain polarized epithelial cells in which influenza virus matures predominantly at the apical surfaces above tight junctions. Influenza virus glycoproteins were found to be localized at the same membrane domain from which the virus budded. When polarized primary AGMK cells were infected with recombinant SV40 viruses containing DNA coding for either an influenza virus H1 or H2 subtype hemagglutinin (HA), the HA proteins were preferentially expressed at the apical surface in a manner identical to that observed in influenza virus-infected cells. Thus, cellular mechanisms for sorting membrane glycoproteins recognize some structural feature of the HA glycoprotein itself, and other viral proteins are not necessary for this process. |
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