Cell biology and life cycle of the testate amoeba Corythion delamarei

Corythion delamarei Bonnet, Thomas, 1960, a typical testate amoeba with hyaline, filiform pseudopodia (Filosea Leidy, 1879), is a mass and widely spread species in the forest soil of Leningrad district. This species has been studied in natural and experimental conditions by means of morphological, cytochemical and morphometrical methods, including original culturing in vitro. The complex life cycle of C. delamarei involves the number of phases: trophozoites, precysts, resting cysts, copulating trophozoits, cystozygotes, cells with spores inside the shell, small amoeboid cells producing spores after germination. Different stages display structural peculiarities reflecting adaptation to exogenous environment. In C. delamarei sexual process has been first discovered. It represents a primitive form of isogamic copulation of morphologically similar trophozoites copulation and results in uninuclear cystozygote formation. The zygote nucleus is a synkaryon meiosis is zygotic, and is accomplished in two steps. Copulation occurs only between two trophozoites of one and the same species. Further zygote development includes its excystation that eventually gives rise to a trophozoite which then undergoes several metagamic divisions resulting in spore formation, thus starting a new generation of trophozoites.