Stable transformation of Pleurotus ostreatus to hygromycin B resistance using Lentinus edodes GPD expression signals |
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Authors: | Irie T Honda Y Hirano T Sato T Enei H Watanabe T Kuwahara M |
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Institution: | Wood Research Institute, Kyoto University, Uji, Japan. |
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Abstract: | It was reported that Pleurotus ostreatus was transformed unstably using recombinant plasmids containing a hygromycin B phosphotransferase gene (hph) under the control of Aspergillus nidulans expression signals, and that the plasmids were maintained extrachromosomally in the transformants. Here we report a stable and integrative transformation of the fungus to hygromycin B resistance, using a recombinant hph fused with Lentinus edodes glyceraldehyde-3-phosphate dehydrogenase expression signals. Restriction-enzyme-mediated integration (REMI) was also tried and increased the transformation efficiency about ten-fold. |
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