Retinal vascular endothelium expresses fibronectin and class II histocompatibility complex antigens in experimental autoimmune uveitis |
| |
| Authors: | L S Fujikawa C C Chan C McAllister I Gery J J Hooks B Detrick R B Nussenblatt |
| |
| Affiliation: | 1. Institut Clinic d’Oftalmologia, Hospital Clínic de Barcelona, Universitat de Barcelona, Barcelona, Spain;2. Fundació Clínic per a la Recerca Biomèdica, IDIBAPS, Barcelona, Spain;1. Ocular Imaging Research and Reading Center (OIRRC), Omaha, Nebraska;2. Department of Ophthalmology, Rush University Medical Center, Chicago, Illinois;3. Palmetto Retina Center, LLC, West Columbia, South Carolina;4. Banker''s Retina Clinic and Laser Center, Navrangpura, Ahmedabad, India;5. Department of Ophthalmology, Pitie-Salpetriere Hospital, University Pierre et Marie Curie, Paris VI, Paris, France;6. Organización Médica de Investigación, Uruguay 725 PB, Buenos Aires, Argentina;7. Department of Ophthalmology, Hokkaido University Graduate School of Medicine, Sapporo, Hokkaido, Japan;8. Aravind Eye Hospital, Madurai, Tamil Nadu, India;9. Department of Ophthalmology, Ludwig-Maximillians-University, Munich, Germany;10. Santen, Inc., Emeryville, California;11. Genentech, Inc., South San Francisco, California;1. The Ritchie Centre, Hudson Institute of Medical Research, 27-31 Wright Street, Clayton 3168, Melbourne, VIC, Australia;2. Department of Obstetrics and Gynaecology, School of Clinical Sciences, Monash University, Monash Medical Centre, 246 Clayton Road, Clayton 3168, Melbourne, VIC, Australia;3. Department of Obstetrics and Gynaecology, Faculty of Medicine, National University of Malaysia, Jalan Yaacob Latiff, Bandar Tun Razak, 56000 Cheras, Kuala Lumpur, Malaysia;4. Department of Medicine, School of Clinical Sciences, Monash University, Monash Medical Centre, 246 Clayton Road, Clayton 3168, Melbourne, VIC, Australia |
| |
| Abstract: | To analyze the role of the retinal vascular endothelial cells in the development of experimental autoimmune uveitis (EAU), we studied the presence of Ia antigen and FN in retinal vessels of Lewis rats immunized with retinal S antigen. Immunopathologic studies were performed on frozen tissues obtained during various stages of the disease. Our results show that Ia antigen was not present in the normal rat retina, and there was very little FN present in a few retinal vessels. One to two days prior to the histologic and clinical onset of EAU, FN was found to be increased in the retinal vessels. Ia antigen was found to be present in the retinal vessels coincident with the first signs of cellular infiltration. During the stage of maximal cellular infiltration, FN was present diffusely throughout the retina, as well as in the subretinal space, and Ia antigen was found diffusely in the cellular infiltrate. Therefore, FN and Ia antigen reflect the immunomodulation of vascular endothelial cells in EAU, which may be very important in the pathogenesis of retinal S antigen-induced uveitis. Two possible mechanisms for the role of the activation of the retinal vascular endothelium in the development of retinal inflammation in uveitis are discussed. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
