| 秋水仙碱诱导梨离体叶片再生多倍体 |
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| 引用本文： | 孙洪雁,孙清荣,辛力. 秋水仙碱诱导梨离体叶片再生多倍体[J]. 西北植物学报, 2009, 29(9) |
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| 作者姓名： | 孙洪雁 孙清荣 辛力 |
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| 作者单位： | 山东省果树研究所,农业部泰安温带果树资源重点野外科学观测试验站,山东省果树生物技术育种重点实验室,山东泰安,271000 |
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| 基金项目： | 山东省优秀中青年科学家科研奖励基金，山东省科技发展计划项目 |
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| 摘 要： | 以二倍体梨(Pyrus communis L.) 品种'丰产'试管苗的离体叶片为外植体,研究了秋水仙碱处理对叶片不定梢再生及多倍体诱导率的影响.结果表明:(1)随秋水仙碱处理时间增加,叶片不定梢再生率下降,而多倍体诱导率增加;以0.4%的秋水仙碱溶液处理叶片48 h效果最好,多倍体诱导率为6.1%.(2)流式细胞仪(Flow cytometry)分析鉴定表明,获得的多倍体有三倍体、四倍体和混倍体;多倍体与二倍体的形态特征差异明显,多倍体比二倍体茎粗、节间短,叶形指数小,根粗而且短.
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| 关 键 词： | 梨 离体叶片 秋水仙碱 多倍体 |
In vitro Colchicine-induced Polyploid Plantlets from Leaf Explants of Pear(Pyrus communis L.) |
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| SUN Hong-yan,SUN Qing-rong,XIN Li. In vitro Colchicine-induced Polyploid Plantlets from Leaf Explants of Pear(Pyrus communis L.)[J]. Acta Botanica Boreali-Occidentalia Sinica, 2009, 29(9) |
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| Authors: | SUN Hong-yan SUN Qing-rong XIN Li |
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| Affiliation: | SUN Hong-yan,SUN Qing-rong,XIN Li(The State Agriculture Ministry Experiment Station of Temperate Fruits Germplasm Key Outdoor Observation in Taian,Sh,ong Fruits Biotechnology Breeding Key Lab,Sh,ong Institute of Pomology,Taian,Sh,ong 271000,China) |
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| Abstract: | In vitro leaves of diploid pear(Pyrus communis L.) cultivar ' Fertility' were used as explants.Effect of colchicine treatment on shoot regeneration and polyploid production was examined.With the increase of colchicine treatment time,shoot regeneration frequency decreased and polyploid production rate increased.The highest polyploid regeneration rate(6.1%) was obtained when leaf explants were incubated in 0.4% colchicine solution for 48 h.The ploidy levels in putative polyploid individuals analysed by flow c... |
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| Keywords: | Pyrus communis leaf in vitro colchicine polyploid |
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