Archaeal promoter-directed expression of the Halobacterium salinarum catalase-peroxidase gene |
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| Authors: | S Long M L Salin |
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| Institution: | (1) Department of Biochemistry and Molecular Biology, Mississippi State University, Mississippi State, MS 39762, USA Tel. +1-662-3252763; Fax +1-662-3258664 e-mail: mls1@ra.msstate.edu, US |
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| Abstract: | The Halobacterium salinarum catalase-peroxidase gene was subcloned into shuttle vectors pWL102 and pWL202 and expressed under the control of different
archaeal promoters. When Hbt. salinarum was transformed with the catalase-peroxidase gene under the control of its own promoter, catalase-peroxidase activity increased
twofold. Catalase-peroxidase activity increased threefold when Hbt. salinarum was transformed with the catalase-peroxidase gene under the control of a tRNA promoter. This bifunctional enzyme in Hbt. salinarum was not induced by environmental stresses such as H2O2, intense light, darkness, high temperature, low temperature, redox inhibitors, heavy metals, or ions.
Received: May 5, 2000 / Accepted: August 28, 2000 |
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| Keywords: | Bifunctional enzyme Induction Oxidative stress Polymerase chain reaction |
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