Death rate in a small air-lift loop reactor of vero cells grown on solid microcarriers and in macroporous microcarriers |
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| Authors: | D E Martens E A A Nollen M Hardeveld C A M van der Velden-de Groot C D de Gooijer E C Beuvery J Tramper |
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| Institution: | (1) Department of Food Science, Food and Bioengeneering Group, Agricultural University, Bomenweg 2, 6703 HD Wageningen, The Netherlands;(2) Department of Radiobiology, University of Groningen, Bloemsingel 1, Groningen, The Netherlands;(3) Laboratory of Inactivated Viral Vaccines, National Institute of Public Health and Environmental Protection, P.O. Box 1, 3720 BA Bilthoven, The Netherlands |
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| Abstract: | The death rate of Vero cells grown on Cytodex-3 microcarriers was studied as a function of the gas flow rate in a small air-lift loop reactor. The death rate may be described by first-order death-rate kinetics. The first-order death-rate constant as calculated from the decrease in viable cells, the increase in dead cells and the increase in LDH activity is linear proportional to the gas flow rate, with a specific hypothetical killing volume in which all cells are killed of about 2·10–3 m3 liquid per m3 of air bubbles. In addition, an experiment was conducted in the same air-lift reactor with Vero cells grown inside porous Asahi microcarriers. The specific hypothetical killing volume calculated from this experiment has a value of 3·10–4 m3 liquid per m3 of air bubbles, which shows that the porous microcarriers were at least in part able to protect the cells against the detrimental hydrodynamic forces generated by the bubbles. |
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| Keywords: | air lift macroporous microcarrier shear sparging Vero cell |
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