Environmental optimization for bioconversion of triolein into 7,10-dihydroxy-8(<Emphasis Type="Italic">E</Emphasis>)-octadecenoic acid by <Emphasis Type="Italic">Pseudomonas aeruginosa</Emphasis> PR3 |
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Authors: | In-Ae Chang Jae-Han Bae Min-Jung Suh In-Hwan Kim Ching T Hou Hak-Ryul Kim |
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Institution: | (1) Department of Animal Science and Biotechnology, Kyungpook National University, Daegu, South Korea, 702-701;(2) Department of Food and Nutrition, College of Health Science, Korea University, Seoul, South Korea;(3) Microbial Genomic and Bioprocessing Research Unit, National Center for Agricultural Utilization Research, ARS, USDA, Peoria, IL, USA |
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Abstract: | Hydroxy fatty acids (HFAs), originally found in small amount mainly from plant systems, are well known to have special properties
such as higher viscosity and reactivity compared with other normal fatty acids. Recently, various microbial strains were tested
to produce HFAs from different unsaturated fatty acids. Among those microbial strains tested, Pseudomonas aeruginosa PR3 are well known to utilize various unsaturated fatty acids to produce mono-, di-, and tri-HFAs. Previously, we reported
that strain PR3 could utilize triolein as a substrate for the production of 7,10-dihydroxy-8(E)-octadecenoic acid (DOD) via the induction of lipase activity (Chang et al., Appl Microbiol Biotechnol, 74:301–306, 2007). In this study, we focused on the development of the optimal environmental conditions for DOD production from triolein by
PR3. Optimal initial medium pH and incubation temperature were pH 8.0 and 25°C, respectively. Magnesium ion was essentially
required for DOD production. Optimal inoculum size, time for substrate addition, and substrate concentration were 1%, 12 to
24 h, and 300 mg, respectively. |
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Keywords: | Pseudomonas aeruginosa Hydroxy fatty acid Triolein Optimization Bioconversion |
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