Location of a mutation in the aspartyl-tRNA synthetase gene of Escherichia coli K12 |
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| Authors: | G J Sharples R G Lloyd |
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| Affiliation: | Department of Genetics, University of Nottingham, Medical School, Queens Medical Centre, Great Britain. |
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| Abstract: | A mutation (tls-1) that confers a temperature-sensitive growth phenotype in Escherichia coli was shown by DNA cloning and sequencing to be an allele of aspS, the gene for aspartyl-tRNA synthetase. The mutation, which lies near minute 41 on the genetic map, was located some 2.3 kb from the 5' end of the ruvAB operon. A DNA fragment encoding the carboxy-terminus of AspRS was found to be sufficient to allow growth of a tls-1 strain at the non-permissive temperature. |
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