A whole‐genome,radiation hybrid mapping resource of hexaploid wheat |
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Authors: | Vijay K Tiwari Adam Heesacker Oscar Riera‐Lizarazu Hilary Gunn Shichen Wang Yi Wang Young Q Gu Etienne Paux Dal‐Hoe Koo Ajay Kumar Ming‐Cheng Luo Gerard Lazo Robert Zemetra Eduard Akhunov Bernd Friebe Jesse Poland Bikram S Gill Shahryar Kianian Jeffrey M Leonard |
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Institution: | 1. Department of Plant Pathology, Wheat Genetics Resource Center, Kansas State University, Manhattan, KS, USA;2. Department of Crop and Soil Science, Oregon State University, Corvallis, OR, USA;3. Dow AgroSciences, Pullman, WA, USA;4. Crop Improvement and Genetics Research Unit, USDA‐ARS, Albany, NY, USA;5. Diversité et Ecophysiologie des Céréales, INRA, UMR 1095 6. Génétique, Clermont‐Ferrand, France;7. Diversité et Ecophysiologie des Céréales, UMR 1095 8. Génétique, Université Blaise Pascal, Aubière Cedex, France;9. Department of Plant Sciences, North Dakota State University, Fargo, ND, USA;10. Department of Plant Sciences, University of California, Davis, CA, USA;11. Cereal Disease Laboratory, University of Minnesota, Saint Paul, MN, USA |
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Abstract: | Generating a contiguous, ordered reference sequence of a complex genome such as hexaploid wheat (2n = 6x = 42; approximately 17 GB) is a challenging task due to its large, highly repetitive, and allopolyploid genome. In wheat, ordering of whole‐genome or hierarchical shotgun sequencing contigs is primarily based on recombination and comparative genomics‐based approaches. However, comparative genomics approaches are limited to syntenic inference and recombination is suppressed within the pericentromeric regions of wheat chromosomes, thus, precise ordering of physical maps and sequenced contigs across the whole‐genome using these approaches is nearly impossible. We developed a whole‐genome radiation hybrid (WGRH) resource and tested it by genotyping a set of 115 randomly selected lines on a high‐density single nucleotide polymorphism (SNP) array. At the whole‐genome level, 26 299 SNP markers were mapped on the RH panel and provided an average mapping resolution of approximately 248 Kb/cR1500 with a total map length of 6866 cR1500. The 7296 unique mapping bins provided a five‐ to eight‐fold higher resolution than genetic maps used in similar studies. Most strikingly, the RH map had uniform bin resolution across the entire chromosome(s), including pericentromeric regions. Our research provides a valuable and low‐cost resource for anchoring and ordering sequenced BAC and next generation sequencing (NGS) contigs. The WGRH developed for reference wheat line Chinese Spring (CS‐WGRH), will be useful for anchoring and ordering sequenced BAC and NGS based contigs for assembling a high‐quality, reference sequence of hexaploid wheat. Additionally, this study provides an excellent model for developing similar resources for other polyploid species. |
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Keywords: | Radiation hybrid mapping wheat resource
Triticum aestivum
physical mapping genome assembly gamma radiation |
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