Paradoxical effects of amiloride analogs on protein phosphorylation and serotonin release induced by agonists in human platelets |
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| Authors: | K Yoshida R Matoba E J Cragoe V T Nachmias |
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| Affiliation: | Department of Legal Medicine, Osaka University, Medical School, Japan. |
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| Abstract: | We examined the effects of newly exploited amiloride analogs on protein phosphorylation and serotonin secretion induced by various agonists in human platelets. 3', 4'-dichlorobenzamil (DCB) and to a lesser extent, 2', 4'-dimethylbenzamil (DMB), which in many cells highly specific inhibitors of Na+/Ca2+-pump, inhibited the phosphorylation of 47K- and 20K-dalton proteins and serotonin secretion in human platelets independently of the action on the pump. DCB also induced dephosphorylation of 47K and 20K after the phosphorylation of these proteins by thrombin and released serotonin by itself. |
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