|
|||||
|
|
| 纤溶酶产生菌中华根霉12~#的诱变选育 | |
| 引用本文： | 李树文,刘晓兰,杜连祥,路福平,咸洪泉.纤溶酶产生菌中华根霉12~#的诱变选育[J].工业微生物,2010,40(1):50-54. |
| 作者姓名： | 李树文 刘晓兰 杜连祥 路福平 咸洪泉 |
| 作者单位： | 1. 青岛农业大学生命科学学院,青岛,266109 2. 齐齐哈尔大学生命科学与工程学院,齐齐哈尔,161006 3. 天津科技大学,天津,300222 |
| 基金项目： | 黑龙江省自然科学基金资助项目 |
| 摘 要： | 以产纤溶酶菌中华根霉12~#为出发菌株,以SDS为抗性因子,紫外线为诱变剂,通过液体发酵的方式筛选高产菌株。共得到48株SDS抗性突变株,命名为LH-系列。经过筛选,得到了1株遗传稳定、酶活力提高了120%左右的高产菌株LH-45,以及2株具有潜在高产能力的突变株LH-15和LH-28。 |
| 关 键 词： | 中华根霉 纤溶酶 液体发酵 突变株 |
Breeding of fibrinolysin-producing mutants from Rhizopus chinesis 12~# |
|
| LI Shu-wen,LIU Xiao-lan,DU Lian-xiang,LU Fu-ping,XIAN Hong-quan.Breeding of fibrinolysin-producing mutants from Rhizopus chinesis 12~#[J].Industrial Microbiology,2010,40(1):50-54. | |
| Authors: | LI Shu-wen LIU Xiao-lan DU Lian-xiang LU Fu-ping XIAN Hong-quan |
| Institution: | LI Shu-wen, LIU Xiao-lan, DU Lian-xiang, LU Fu-ping, XIAN Hong-quan(1. Qingdao Agricultural University Life Science College, Qingdao 266109; 2. Qiqihar University Life Science and Engineering College, Qiqihar 161006; 3. Tianjin University of Science and Technology, Tianjin 300222 ) |
| Abstract: | Treated with UV, the spores of Rhizopus chinesis 12~# were cultivated in medium containing SDS to get SDS-resistant mutants for high-producing fibrinolytic enzyme. 48 SDS-resistant mutants were obtained by liquid fermentation and named LH-series. After further screened, LH-45, LH-15 and LH-28 obtained were potential high producing mutants and the fibrinolysin activity of LH-45 with good genetic stability increased by 120% or so. |
| Keywords: | Rhizopus chinesis fibrinolysin liquid fermentation mutant |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |