Uptake of Lucifer yellow CH in leaves ofCommelina communis is mediated by endocytosis |
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Authors: | S Hillmer R Hedrich M Robert-Nicoud D G Robinson |
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Institution: | (1) Pflanzenphysiologisches Institut der Universität, Untere Karspüle 2, D-3400 Göttingen, Federal Republic of Germany;(2) Abteilung Molekulare Biologie, Max-Planck-Institut für Biophysikalische Chemie, Göttingen |
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Abstract: | Summary Lucifer yellow CH (LY) uptake into intact leaves ofCommelina communis has been studied with conventional fluorescence microscopy as well as confocal laser scanning microscopy. LY, a highly fluorescent tracer for apoplastic transport in plants and fluid phase endocytosis in animal cells, accumulates in the vacuole of leaf cells. However, considerable differences in the ability to take up LY were observed among the various cell types. Mesophyll cells take up large amounts of the dye whereas epidermal cells, including guard and subsidiary cells, showed no fluorescence in their vacuoles. An exception to this are trichome cells which show considerable accumulation of LY. When introduced into the cytoplasm of mesophyll protoplasts ofC. communis by means of a patch-clamp pipette, LY does not enter the vacuole. This supports the contention that exogenous LY can only gain access to the vacuole via endocytosis. Differences in the capacity for LY uptake may therefore reflect differences in endocytotic activity.Abbreviations CLSM
Confocal laser scanning microscopy
- DIC
differential interference contrast
- LY
Lucifer yellow CH
- PM
plasma membrane |
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Keywords: | Commelina leaves Endocytosis Laser scan Lucifer yellow Mesophyll protoplasts Patch pipette |
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