Inhibition of c-H-ras oncogene induced transformation of rat embryo fibroblasts by cotransfected polynucleotides containing alternating purine-pyrimidine sequences |
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| Authors: | R Banerjee W L Hsiao I B Weinstein D Grunberger |
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| Affiliation: | Institute of Cancer Research and Comprehensive Cancer Center, College of Physicians and Surgeons, Columbia University, New York, NY 10032. |
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| Abstract: | When Rat 6 cultures were cotransfected with an activated c-H-ras oncogene (pT24) and poly(dG-m5dC), a synthetic polymer that has the potential to form Z DNA, there was marked inhibition of cell transformation. Cotransfection of pT24 DNA with poly(dG-dC) caused somewhat less inhibition, poly(dA-dC). (dG-dT) caused moderate inhibition, and poly(dG). (dC) exerted negligible inhibition. Evidence was obtained that the inhibition seen with poly(dG-m5dC) was not simply due to an inhibition of cellular uptake of the pT24 DNA. Our results suggest that certain polymers that have the potential to form Z DNA can inhibit the integration and expression of a transfected oncogene. |
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