Determination of fumonisins and hydrolyzed fumonisin B1 in microbial culture media by LC/ESI-MS |
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| Authors: | E. Vekiru E. Fuchs G. Schatzmayr M. Täubel E. M. Binder R. Krska |
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| Affiliation: | (1) Institute for Agrobiotechnology, Center for Analytical Chemistry, Konrad Lorenz Str. 20, A-3430 Tulln;(2) Erber AG, Industriestrasse 21, A-3130 Herzogenburg;(3) Biomin IAN GmbH, Industriestrasse 21, A-3130 Herzogenburg;(4) Department of Environmental Biotechnology, Institute for Agrobiotechnology Tulln, Konrad Lorenz Str. 20, A-3430 Tulln |
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| Abstract: | In order to analyze liquid growth media for fumonisin B1, B2 and hydrolyzed fumonisin B1 (HFB1) after incubation with microorganisms for degradation studies, a liquid chromatography/electrospray ionisation mass spectrometry (LC/ESI-MS) method was developed. The use of phytosphingosine hydrochloride (PSH) and T-2-toxin-d3 (T2d3) as internal standard has been tested. The detection limit established with PSH as an internal standard was about 20 ng/mL for FB1 and FB2 and about 50 ng/mL for HFB1. The developed method allows the rapid, simultaneous and quantitative determination of these analytes in microbial culture media without any cleanup. Presented at the 25th Mykotoxin Workshop in Giessen, Germany May 19–21, 2003 |
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| Keywords: | LC-ESIMS mycotoxins fumonisins culture media |
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