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Proteins in Tumor-Derived Plasma Extracellular Vesicles Indicate Tumor Origin |
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| Institution: | 1. Department of Medicine, Washington University School of Medicine in St Louis, St Louis, Missouri, USA;2. Siteman Cancer Center, Washington University School of Medicine in St Louis, St Louis, Missouri, USA;3. Department of Surgery, Washington University School of Medicine in St Louis, St Louis, Missouri, USA;4. Department of Pathology and Immunology, Washington University School of Medicine in St Louis, St Louis, Missouri, USA;5. Department of Anesthesiology, Washington University School of Medicine in St Louis, St Louis, Missouri, USA |
| Abstract: | Cancer-derived extracellular vesicles (EVs) promote tumorigenesis, premetastatic niche formation, and metastasis via their protein cargo. However, the proteins packaged by patient tumors into EVs cannot be determined in vivo because of the presence of EVs derived from other tissues. We therefore developed a cross-species proteomic method to quantify the human tumor-derived proteome of plasma EVs produced by patient-derived xenografts of four cancer types. Proteomic profiling revealed individualized packaging of novel protein cargo, and machine learning accurately classified the type of the underlying tumor. |
| Keywords: | exosomes extracellular vesicles proteomics patient-derived xenografts cancer ProteoClade machine learning CRC"} {"#name":"keyword" "$":{"id":"kwrd0050"} "$$":[{"#name":"text" "_":"colorectal cancer DB"} {"#name":"keyword" "$":{"id":"kwrd0060"} "$$":[{"#name":"text" "_":"digestion buffer DLS"} {"#name":"keyword" "$":{"id":"kwrd0070"} "$$":[{"#name":"text" "_":"dynamic light scattering EV"} {"#name":"keyword" "$":{"id":"kwrd0080"} "$$":[{"#name":"text" "_":"extracellular vesicle FA"} {"#name":"keyword" "$":{"id":"kwrd0090"} "$$":[{"#name":"text" "_":"formic acid LDA"} {"#name":"keyword" "$":{"id":"kwrd0100"} "$$":[{"#name":"text" "_":"linear discriminant analysis LFQ"} {"#name":"keyword" "$":{"id":"kwrd0110"} "$$":[{"#name":"text" "_":"label-free quantitation MS"} {"#name":"keyword" "$":{"id":"kwrd0120"} "$$":[{"#name":"text" "_":"mass spectrometry PDAC"} {"#name":"keyword" "$":{"id":"kwrd0130"} "$$":[{"#name":"text" "_":"pancreatic ductal carcinoma PDX"} {"#name":"keyword" "$":{"id":"kwrd0140"} "$$":[{"#name":"text" "_":"patient-derived xenograft RT"} {"#name":"keyword" "$":{"id":"kwrd0150"} "$$":[{"#name":"text" "_":"room temperature |
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