ARHGEF15 is expressed in undifferentiated spermatogonia but is not required for spermatogenesis in mice |
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| Institution: | 1. Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China;2. University of Chinese Academy of Sciences, Beijing 100049, China;3. Qinghai Key Laboratory of Animal Ecological Genomics, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China;1. Department of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowski 1A, 10-719, Olsztyn, Poland;2. Department of Human Histology and Embryology, School of Medicine, Collegium Medicum, University of Warmia and Mazury in Olsztyn, Warszawska 30, 10-082, Olsztyn, Poland;1. Department of Theriogenology, Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt;2. Department of Theriogenology, Faculty of Veterinary Medicine, Benha University, Egypt;3. Department of Animal Reproduction and A.I, Veterinary Research Division, National Research Centre, Dokki, 12622 Giza, Egypt;4. Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi 1677-1, Japan;5. Global Agromedicine Research Center (GAMRC), Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080 8555, Japan;1. Department of Zoology, Faculty of Science, Al-Azhar University, Nasr City, Egypt;2. Department of Zoology, Faculty of Science, Cairo University, Giza, Egypt;1. Medical Genetics & Genomics Laboratories, Magee-Womens Hospital of UPMC, Pittsburgh, PA, Unitetd States;2. Department of Obstetrics, Gynecology and Reproductive Sciences, University of Pittsburgh School of Medicine, Pittsburgh, PA, Unitetd States;3. Medical Genetics, Children''s Hospital of Pittsburgh of UPMC, Pittsburgh, PA, Unitetd States;4. Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA, Unitetd States;5. Department of Human Genetics, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, PA, Unitetd States;6. Magee-Womens Research Institute, Pittsburgh, PA, Unitetd States |
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| Abstract: | Spermatogenesis is a continual process that relies on the activities of undifferentiated spermatogonia, which contain spermatogonial stem cells (SSCs) that serve as the basis of spermatogenesis. The gene expression pattern and molecular control of fate decisions of undifferentiated spermatogonia are not well understood. Rho guanine nucleotide exchange factor 15 (ARHGEF15, also known as EPHEXIN5) is a guanine nucleotide-exchange factor (GEF) that activates the Rho protein. Here, we reported that ARHGEF15 was expressed in undifferentiated spermatogonia and spermatocytes in mouse testes; however, its deletion did not affect spermatogenesis. Arhgef15-/- mice were fertile, and histological examination of the seminiferous tubules of Arhgef15-/- mice revealed complete spermatogenesis with the presence of all types of spermatogenic cells. Proliferation and differentiation of the undifferentiated spermatogonia were not impacted; however, further analysis showed that Arhgef15 deletion resulted in decreased expression of Nanos2, Lin28a and Ddx4. Together, these findings suggest that ARHGEF15 was specifically enriched in undifferentiated spermatogonia and regulated gene expression but dispensable for spermatogenesis in mice. |
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| Keywords: | ARHGEF15 Spermatogenesis Undifferentiated Spermatogonia Fertility Spermatogonial Stem Cells |
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