Differential expression of desmin in the uterine myometrium and cervix as a possible mechanism for successful parturition in rats |
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| Affiliation: | 1. Departamento de Fisiología y Desarrollo Celular, Instituto Nacional de Perinatología “Isidro Espinosa de los Reyes”, México;2. Coordinación de Salud Mental, Instituto Nacional de Perinatología “Isidro Espinosa de los Reyes”, México;3. Unidad de Investigación en Reproducción Humana, Instituto Nacional de Perinatología-Facultad de Química, Universidad Nacional Autónoma de México, México;1. Division of Animal Science, ICAR-Central Island Agricultural Research Institute, Port Blair 744105, Andaman and Nicobar Islands, India;2. Animal Physiology and Reproduction, ICAR-National Research Centre on Mithun, Medziphema 797106, Nagaland, India;3. Division of Animal Physiology, ICAR-National Dairy Research Institute, Karnal 13200, Haryana, India;1. The Laboratory of Cell and Genetics, the People’s Hospital of Hebi, 458030 Hebi City, Henan, China;2. Tianjin Key Laboratory of Exercise Physiology and Sports Medicine, College of Exercise and Health Sciences, Tianjin University of Sport, Tianjin City 301617, China;3. Department of Obstetrics and Gynecology, the People’s Hospital of Hebi, 458030 Hebi City, Henan, China;4. Eproductive Center, the People’s Hospital of Hebi, 458030 Hebi City, Henan, China;5. Sumy National Agrarian University, Sumy City 40021, Ukraine;1. Department of Gyanecology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shang hai 201620, PR China;2. Department of Gynaecology, Kunshan Hospital of Traditional Chinese Medicine Affiliated to Yangzhou University, Kunshan, Jiangsu 215300, PR China |
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| Abstract: | Expression of desmin, an intermediate filament, in the myometrium and cervix were investigated in peripartum rats (full term day 22 of pregnancy (DP22)). Des mRNA was expressed in lesser amounts in the cervix at peripartum (DP17 and 21, and day of birth 1 (DB1)), compared to those in the cervixes of ovariectomized rats. Immunohistochemical analysis revealed that desmin protein was diffusely present in the myometrium, and locally in the epithelium of the cervix. Western blot analysis showed that desmin protein levels in the myometrium increased 4- to 6-fold at DP17, 21 and DB1, and decreased rapidly at DB2 to the basal level observed in ovariectomized or non-pregnant rats. In contrast, cervical desmin protein levels increased approximately 10-fold at DP21 compared to those in ovariectomized rats, but decreased rapidly at DB1, indicating its decrease at parturition and an inconsistency between mRNA and protein expression. The administration of 17β-estradiol to ovariectomized rats increased desmin protein levels in the myometrium and cervix after 24 h. S-nitrosylated desmin protein was detected in the myometrium and cervix at DP21. The mRNA expression of inducible nitric oxide synthase was consistent with the expression of desmin protein. Thus, desmin, which is regulated by estradiol, is differentially expressed in the myometrium and cervix at peripartum possibly for successful pregnancy and parturition. In the cervix, desmin protein expression seems to be regulated by estradiol at the translational level. S-nitrosylation of desmin may have a potential role in the peripartum uterus. |
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| Keywords: | Desmin Myometrium Cervix Parturition Peripartum |
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