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The use of antibodies against DNA modified by trans-diamminedichloroplatinum for identification of specific DNA sequences |
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| Authors: | V I Kiseleva M F Turchinski? A M Poverenny? |
| Abstract: | DNA modified by trans-diamminedichloroplatinum (trans-DDP) is suggested as an effective probe for non-isotopic hybridisation. Highly specific anti trans-DDP-DNA antibodies and peroxidase-conjugated antibodies to antirabbit Ig and protein A system have been used for the hybrids visualisation. This method allows one to detect 2 pg/mm2 DNA. |
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