Molecular characterization and assessment of genetic diversity of inbred lines showing variability for drought tolerance in maize |
| |
Authors: | T Nepolean Ishwar Singh Firoz Hossain Neha Pandey H S Gupta |
| |
Institution: | 1. Maize Genetics Unit, Division of Genetics, Indian Agricultural Research Institute, New Delhi, 110012, India 2. Directorate of Maize Research, PUSA, New Delhi, 110012, India
|
| |
Abstract: | A set of 24 genotypes bred at different centres in India as well as in CIMMYT showing variability for drought tolerance were selected for molecular and morpho-physiological characterization. A set of 35 SSR markers, having genome-wide coverage, was chosen for genotyping the inbreds. These markers generated a total of 111 polymorphic alleles with an average of 3.17 alleles per locus. The minimum and maximum PIC value was 0.27 and 0.77 with a mean of 0.5. A total of 13 unique alleles were found in the 24 inbred lines. The coefficient of genetic dissimilarity ranged from 0.192 to 0.803. NJ-based tree suggested the presence of three major clusters of which, two of them had subgroups. Phenotyping of inbreds by morpho-physiological traits revealed that there was a positive relationship among root length, chlorophyll content, relative water content while anthesis-silking interval was negative relationship with all these traits. Genotyping data complemented by morpho-physiological parameters were used to identify a number of pair-wise combinations for the development of mapping population segregating for drought tolerance and potential heterotic pairs for the development of drought tolerant hybrids. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|