Estimation of cell DNA synthesis rate from flow-cytometric histograms |
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Authors: | A. Bertuzzi A. Gandolfi A. Germani R. Vitelli |
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Affiliation: | 1. Istituto di Analisi dei Sistemi ed Informatica del CNR, Viale Manzoni 30, 00185, Roma, Italy
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Abstract: | The rate of DNA synthesis along S-phase can be estimated on the basis of a simple model of a cell population, from a single FCM histogram under conditions of balanced exponential growth, and from two histograms suitably spaced in time under conditions of locally exponential S-phase influx. An algorithm that constructs a piecewise constant approximation of the synthesis rate and estimates the phase fractions from a single histogram is presented. The results of the application of the algorithm to a set of computer generated histograms in balanced exponential growth are reported. |
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