Biocatalysis using microemulsion-based polymer gels containing lipase |
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| Institution: | 1. Key Laboratory of Synthetic and Biological Colloids, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi, 214122, PR China;2. Polymer Research Institute, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu, 610065, PR China;1. Centro de Investigaciones de Plagas e Insecticidas (UNIDEF/CITEDEF/CONICET), San Juan Bautista de La Salle 4397, B1603ALO-Villa Martelli-Buenos Aires, Argentina;2. Departamento de Química Física I-Universidad Complutense de Madrid, Ciudad Universitaria s/n, 28040 Madrid, Spain;3. Instituto Pluridisciplinar-Universidad Complutense de Madrid, Avd. Juan XXIII, 28040 Madrid, Spain;1. Institute of Physical and Theoretical Chemistry, University of Regensburg, 93040 Regensburg, Germany;2. Institut de Chimie Séparative de Marcoule, UMR 5257 (CEA/CNRS/UM/ENSCM), 30207 Bagnols sur Cèze, France;1. Key Laboratory of Enhanced Heat Transfer and Energy Conservation of Education Ministry, School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510640, China;2. State Key Laboratory of Subtropical Building Science, South China University of Technology, Guangzhou 510640, China |
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| Abstract: | Natural gelling agents such as gelatin, agar and κ-carrageenan have been tested for the formation of lecithin microemulsion-based gels as well as hydrogels (without surfactant and oil). The results presented in this work provide information concerning the utility of these solid gels as lipase immobilization matrices. It was found that lipase from Pseudomonas cepacia keeps its catalytic function after entrapment in the gels, catalyzing the esterification reaction of propanol with lauric acid in various hydrocarbons at room temperature. Various parameters which affect the lipase catalytic behavior such as the nature and the concentration of the gelling agent, as well as the concentration of the biocatalyst and the mole ratio of the substrates have been examined. High yields (80%) were obtained with agar and κ-carrageenan organogels in isooctane. The remaining lipase activity, in repeated syntheses was found to depend on the nature of the biopolymer used for the formation of the organogels. Gelatin and agar microemulsion-based gels showed the highest operational stability. |
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