A rapid radiometric assay for dihydrofolate reductase |
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| Authors: | R Hayman R McGready M B Van der Weyden |
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| Affiliation: | Department of Medicine, Monash University, Alfred Hospital, Prahran, 3181, Victoria, Australia |
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| Abstract: | A rapid radiochemical procedure for the measurement of dihydrofolate reductase activity is described. The method employs separation of the radiolabeled substrate from the products of the reaction by precipitation with acetic acid and zinc sulfate. This method permits rapid processing of samples and climinates time-consumlng column chromatography techniques. Good agreement of results is obtained when the radioactive method is compared to the spectrophotometric assay method. |
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