Adaptations of Goldner's Masson Trichrome Stain for the Study of Undecalcified Plastic Embedded Bone |
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| Authors: | Helen E. Gruber |
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| Affiliation: | a Department of Pediatrics, Medical Genetics-Birth Defects Center, Los Angeles, Californiab Department of Pediatrics, University of California, Los Angeles, California |
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| Abstract: | Specialized adaptations for application of Goldner's Masson trichrome stain to plastic embedded undecalcified bone specimens are presented. This stain can be used successfully on methyl-glycol methacrylate, glycol methacrylate and Spurr embedded bones. The stain affords the advantage of good cellular staining due to the hematoxylin component with concomitant sharp discrimination of mature bone matrix which stains green, immature new bone matrix which stains red, and calcified cartilage which stains very pale green. Use of red filters during photomicrography aids in bone-osteoid discrimination in black and white photographs. |
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| Keywords: | bone histology Goldner's stain Masson-Goldner's trichrome stain osteoid |
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