| 狭叶小金梅草组织培养与快速繁殖 |
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| 引用本文： | 李洪林,钟志祥,谢娟,胡光万.狭叶小金梅草组织培养与快速繁殖[J].亚热带植物科学,2020(2):145-147. |
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| 作者姓名： | 李洪林 钟志祥 谢娟 胡光万 |
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| 作者单位： | 中国科学院武汉植物园 |
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| 基金项目： | 中国科学院中-非联合研究中心项目(SAJC201614)资助。 |
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| 摘 要： | 以狭叶小金梅草(Hypoxis angustifolia)当年花苞为材料进行离体快速繁殖体系研究。结果表明,花苞的愈伤诱导适宜培养基为MS+6-BA 0.8 mg·L^-1+NAA 0.05 mg·L^-1+TDZ 0.1 mg·L^-1;诱导芽培养基为MS+6-BA 0.5 mg·L^-1+NAA 0.02 mg·L^-1+AC(活性碳)0.2 g·L^-1;芽继代增殖培养基为MS+6-BA 1.0 mg·L^-1+NAA 0.1 mg·L^-1+花宝1号1 g·L^-1+AC 0.2 g·L^-1,30 d为一个继代周期,增殖系数达10~20倍;壮苗生根培养基为1/2MS+IBA 0.5 mg·L^-1+NAA 0.02 mg·L^-1+AC 0.5 g·L^-1。将生根苗移栽至泥土:蛭石按3:1配制旳基质中,盖膜保湿,成活率达80%。
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| 关 键 词： | 狭叶小金梅草 花蕾 增殖 离体培养 |
In vitro Culture and Rapid Propagation of Hypoxis angustifolia |
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| LI Hong-lin,ZHONG Zhi-xiang,XIE Juan,HU Guang-wan.In vitro Culture and Rapid Propagation of Hypoxis angustifolia[J].Subtropical Plant Science,2020(2):145-147. |
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| Authors: | LI Hong-lin ZHONG Zhi-xiang XIE Juan HU Guang-wan |
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| Institution: | (Wuhan Botanical Garden,Chinese Academy of Sciences,Wuhan 430074,Hubei China) |
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| Abstract: | The buds of Hypoxis angustifolia were used as material for tissue culturing.The results showed that the optimum culture medium for bud callus induction was MS+6-BA 0.8 mg·L^-1+NAA 0.05 mg·L^-1+TDZ 0.1 mg·L^-1;the culture medium for bud induction was MS+6-BA 0.5 mg·L^-1+NAA 0.02 mg·L^-1+AC 0.2 g·L^-1;the culture medium for propagation was MS+6-BA 1.0 mg·L^-1+NAA 0.1 mg·L^-1+Huabao-11 g·L^-1+AC 0.2 g·L^-1.And the subculture circle was 30 d,the multiplication rate was 10-20.The culture medium for root was 1/2 MS+IBA 0.5 mg·L^-1+NAA 0.02 mg·L^-1+AC 0.5 g·L^-1.The suitable transplanting medium was surface soil and vermiculite(3:1),the survival rate reached 80%. |
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| Keywords: | Hypoxis angustifolia bud propagation tissue culture |
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