苏州东山茶树种质资源遗传多样性的ISSR分析

利用ISSR分子标记对苏州洞庭地区的51份茶树(Camellia sinensis)种质资源进行遗传关系研究。结果表明，从12条引物中筛选出6条引物，共扩增出76个位点，其中多态性位点70个，占92.11%。51份茶树种质资源遗传相似性(GS)变化范围0.37～0.89；其中白沙和东灵1号GS值最大、遗传相似程度最高、遗传距离最近。通过类平均聚类(UPGMA)法，可将51份材料分为2个大类，每一大类又分为4个亚组，相同亚组下的亲缘关系较近。其中，部分来自同一品种的不同品系具有较高的遗传相似性系数。ISSR标记可有效评价苏州茶树种质的遗传多样性，为更有效地保护和利用茶树种质资源和茶树优良品种的选育提供遗传信息。

Genetic Diversity Analysis of Camellia sinensis in Dongshan,Suzhou by ISSR

The genetic diversity of tea plant in Dongting district of Suzhou was examined by ISSR. The results showed that 76 bands were amplified with 6 ISSR primers, the polymorphic bands of which were 70, and the polymorphism percentage was 92.11%. The genetic similarity was from 0.37-0.89. The GS was the greatest between Baisha and No.1 Dongling, and they had the highest genetic similarity and the smallest genetic distance. Cluster analysis classified the 51 cultivars into 2 major groups by UPGMA. Each group was divided into four subgroups and the genetic relationship was closer in the same subgroups. Among them, some different strains from the same breed had higher genetic similarity coefficients. The results suggested that ISSR was a useful tool for molecular genetic diversity and relationship analysis of tea cultivars.