苜蓿丫纹夜蛾核型多角体病毒囊膜内核衣壳排列图式的电镜观察

核型多角体病毒有单核衣壳包埋型和多核衣壳包埋型之分，单核衣壳包埋型是在一个病毒囊膜内只包含一个核衣壳，而多核衣壳包埋型的特点是在一个病毒囊膜内包含有２个以上的核衣壳，由于多个核衣壳成束地被包装在同一个病毒囊膜内，又称病毒束［１，２］。Ｈｕｎｔｅｒ等表明在干果斑螟核型多角体病毒中，病毒囊膜内包含２～２３个核衣壳［３］。Ｆｒａｓｅｒ将苜蓿丫纹夜蛾核型多角体病毒接种于秋粘虫细胞系，超薄切片电镜观察，病毒囊膜内包含的核衣壳数变动于２～１７粒，但未研究其核衣壳在病毒囊膜内的排列结构［４］。本研究用苜蓿丫纹…

Electron Microscopic Observation on the Arrangement Patterns of Nucleocapsids within the Envelopes of Autographa californica Nuclear Polyhedrosis Virus

The arrangement patterns of nucleocapsids within the envelope of %Autographa californica% nuclear polyhedrosis virus were studied by electron microscopy. Numbers of nucleocapsids observed in an envelope in their cross sections ranged from 1 to 17, and the frequencies at 2,3,4,5,6 and 7 nucleocapsids were significantly higher than the others, suggesting that these numbers of nucleocapsids were more commonly involved in an envelope. The regular arrangement patterns of nucleocapsids within envelope were observed in cross sections. Envelope outlines can be classified into a triangle (3 nucleocapsids in an envelope),lozenge and square (4 nucleocapsids in an envelope), trapeziun (5 nucleocapsids in an envelope), pentagons (5 or 8 nucleocapsids in an envelope) and hexagons (7 or 10 nucleocapsids in an envelope). The irregular arrangement patterns of nucleocapsids within envelopes were also observed in cross sections.