油桐尺蠖单粒包埋核型多角体病毒（BusuNPV）BamHI—H片段的序列分析

对油桐尺蠖单粒包埋核型多用体病毒（Buzurasuppressariasingle－nucleocapsidnucleopolyhedrovirus，BusuNPV）基因组中BamHI－H片段的序列进行分析，该片段全长2422bp，包括三个开放阅读框：p47基因（AcMNPVORF40的同源区）的5′端，完整的组织蛋白酶基因（cathepsin）（AcMNPVORF127的同源区）和p74基因（AcMNPVORF138的同源区）的3′端。序列比较分析表明，BusuNPV的这三个基因与其它杆状病毒的同源基因具有相同的结构保守区。BusuNPV基因组BamHI－H片段上这三个基因的排列顺序完全不同于AcMNPV相应基因的排列顺序。

Genetic Organization of the BamHI-H Region of the Single Nucleocapsid Nucleopolyhedrovirus of Buzura suppressaria (BusuNPV )

In order to investigate the genomic organization of the single-nucleocapsid nucleopolyhedrovirus (SNPV) of Buzura suppressaria (BusuNPV), the BamHI-H fragment located at mapunits (mu)81. 6-83. 6 of the viral genome was sequenced. The fragment contained two partialand one complete open reading frames (ORFs) representing the 5' end of a p47 gene, a cathepsingene and the 3' end of a p74 gene. Sequence analysis futher revealed that these ORFs have thesame conserved motifs and gene structure as those observed in their homologues from other baculoviruses. The genomic arrangement of the ORFs in the BusuNPV BamHI-H fragment is verydifferent from the arrangement of their homologues in the genome of Autographa californica mutiple nucleocapsid (M) NPV.