Novel method for cell immobilization and its application for production of oligosaccharides from sucrose |
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Authors: | Shin H T Park K M Kang K H Oh D J Lee S W Baig S Y Lee J H |
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Affiliation: | Faculty of Life Science and Technology, Sungkyunkwan University, Suwon, Kyunggi-do, Korea. |
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Abstract: | AIMS: The purpose of the present investigation was to develop a novel method for cell immobilization. METHODS AND RESULTS: Aureobasidium pullulans cells were mixed with an alginate solution, and the mixture was extruded to form small gel beads as hydrated-immobilized cells. The beads were then placed at -15 degrees C for 6-24 h to induce freeze-dehydration. The freeze-dehydration resulted in shrinkage of beads as a result of water removal reducing bead volume by 82% and bead weight by 85%. The dehydrated beads were successfully used for the production of fructo-oligosaccharides in a model reactor system. CONCLUSIONS: Dehydrated beads may provide some commercial advantages over conventional immobilized cells. SIGNIFICANCE AND IMPACT OF THE STUDY: This study shows that bioreactor performance can be improved up to two times by the use of the dehydrated beads. |
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Keywords: | alginate bead bioreactor bioreactor productivity freeze-dehydration fructo-oligosaccharides |
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