白腐菌混合菌降解木质素最佳条件的优化

通过正交试验对3种白腐菌混合菌降解竹材木质素的条件进行优化,结果表明,在温度为32℃、pH3.0、固体发酵时间20 d、培养液与竹材基质质量百分比110%时降解木质素的效率最高.在此基础上,研究了两种诱导剂对白腐菌混合菌降解木质素的影响.结果表明,两种诱导剂均能促进木质素的降解,其中H_2O_2在浓度1%时,木质素降解率高达62.9%,苯甲酸在浓度0.1%时,木质素降解率最高,为67.8%.

Optimization of the Conditions for White-rot Fungus Mixed Bacteria Degrading Lignin

In this paper, the conditions for White-rot fungus mixed bacteria degrading lignin were optimized through the orthogonalexperiments.The results showed that when the temperature was 32℃, pH30, the solid-state fermentation time was 20 d, the culture medium and the substrate quality bamboo was 110%, and the efficiency of lignin degradation was up to a maximum.On this basis, the impact of two kinds of inducing agents acting on mixed white rot fungi degrade lignin was studied.The results showed that two kinds of inducing agents can promote the degradation of lignin, in which the concentration of H_2O_2 at 1%, lignin degradation rate as high as 62.9%, benzoic acid in the concentration of 0.1%, and the highest rate of lignin degradation, to 67.8%.