The bulk of ATP is associated to proteins in the living cell: a release kinetics study |
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Authors: | T Koszegi M Kellermayer E Berenyi K Jobst C F Hazlewood |
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Institution: | Department of Clinical Chemistry, University Medical School, Pecs, Hungary. |
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Abstract: | The analysis of the nonionic detergent induced release kinetics of intracellular components is a promising approach to obtain information on the possible molecular interactions inside the living cell. The molecular interactions of ATP were studied by monitoring the nonionic detergent-induced release kinetics of ATP from cultured human cells. The observed rapid mobilization rate in the presence of Triton X 100 and the retarded release in the presence of Brij 58 were in good agreement with the detergent-induced release kinetics of proteins and K+ reported earlier. These data are consistent with the idea that the bulk of ATP is associated to the protein macromolecules of the living cell. |
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