Inhibition of microtubule assembly is a possible mechanism of action of mitoxantrone |
| |
| Authors: | C K Ho S L Law H Chiang M L Hsu C C Wang S Y Wang |
| |
| Institution: | Department of Medical Research, Veterans General Hospital, Taipei, Republic of China. |
| |
| Abstract: | We have found that mitoxantrone can inhibit the polymerization of brain tubulin in a dose dependent manner. MXT had relatively high affinity for tubulin but had no appreciable effect on tubulin associated guanosine-triphosphatase (GTPase) activity nor could it compete with vinblastine (VB) and colchicine (Col) for tubulin binding sites. Furthermore, MXT (0.1-10 microM) is antiproliferative to cold-treated (0 degree C) epithelial cells after only brief exposure (30 min). These results indicated that MXT is a microtubule inhibitory agent and can exert its anticellular effect through modulation of microtubule assembly. |
| |
| Keywords: | |
|
|
