Hydrophobic properties of Providencia stuartii and other Gram-negative bacteria measured by hydrophobic interaction chromatography |
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| Authors: | N. ISMAEEL,J. R. FURR,W. J. PUGH,A. D. RUSSELL &dagger |
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| Affiliation: | King Abdul-Aziz University, Faculty of Medicine, Department of Clinical Microbiology, PO Box 9029, Jeddah 21413, Saudi Arabia;*Pharmaceutical Microbiology Research Laboratory, Welsh School of Pharmacy, University of Wales Institute of Science and Technology, PO Box 13, Cardiff CF1 3XF, Wales, UK |
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| Abstract: | A hydrophobic interaction chromatography (HIC) procedure was used to compare the relative surface hydrophobicity of three Providencia stuartii strains and wild type and envelope mutants of Escherichia coli and Pseudomonas aeruginosa. Providencia stuartii strain Pv2 adsorbed to a greater extent to octyl- and phenyl-Sepharose than did Pv67. The HIC technique showed a significant difference in surface hydrophobicity between wild type and envelope mutant strains, the latter being considerably more hydrophobic. Pre-treatment of cell suspensions with chlorhexidine produced further changes in the hydrophobic nature of all the strains. Moreover, HIC provides a convenient and rapid alternative means of screening strains for a property potentially associated with adhesiveness. |
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