H.pylori抗体鸡蛋制品对H.pylori感染BALB/c小鼠的实验研究

目的通过幽门螺杆菌(Helicobacter pylori,H.pylori)标准株的接种,建立BALB/c小鼠感染H.pylori胃炎动物模型,评价H.pylori抗体鸡蛋制品对小鼠感染性胃炎的预防效果。方法将灭活的H.pylori国际标准菌株(NCTC 11637)作为抗原,对产蛋鸡进行免疫。免疫后收集鸡蛋,对达到效价的鸡蛋,无菌采集卵黄。BALB/c小鼠60只,适应性喂养1周后,随机分为5组,Ⅰ组为胃炎模型组,Ⅱ组为生理盐水组,Ⅲ、IV、V组分别为低剂量、中剂量和高剂量卵黄抗体组,每组12只。Ⅰ组予H.pylori菌液灌胃造模,Ⅱ组先予生理盐水灌胃后再予H.pylori菌液灌胃对照,Ⅲ、IV、V组分别用不同剂量抗H.pylori卵黄抗体灌胃后再予H.pylori菌液灌胃造模。小鼠均于距最后一次灌胃后8周全部处死,用微需氧细菌培养检测H.pylori定植;HE染色观察小鼠胃黏膜病理组织学改变。结果在接种H.pylori后第8周,Ⅰ组和Ⅱ组小鼠胃内均有大量H.pylori定植,感染率为91.7%,Ⅲ组的感染率是58.3%,IV和V组的感染率均小于30%。结论 H.pylori抗体鸡蛋制品可以抑制BALB/c小鼠感染H.pylori,抗体的保护作用与抗体的剂量呈正相关。

Effect of egg-yolk antibody on experimental Helicobacter pylori infection in BALB/c mice

Objective To establish the model of BALB/c mice infected by Helicobacter pylori（H.pylori）,and evaluate the preventive effect of egg-yolk antibody on infective gastritis（IG）.Method Laying hens were immunized with inactivated vaccine from H.pylori type strain（NCTC 11637）.When the antibody titer of eggs was enough,the yolks were collected sterilely.60 BALB/c mice were divided randomly into five groups after fed normally for one week.The mice in groupⅠwere inoculated with H.pylori in order to establish H.pylori IG.The mice in group Ⅱ were inoculated with normal saline and then with H.pylori.The mice in group III,IV and Ⅴ were inoculated respectively with antibody in different doses and then with H.pylori.After 8 weeks,all mice were sacrificed.The infection of H.pylori in gastric mucosa of the mice was analyzed by culture method.The histopathological changes in the gastric mucosa of mice were assessed by H-E staining.Result There were more H.pylori in the stomach on the 8th week after inoculation and the infection rate was 91.7% in groupⅠand Ⅱ.The infection rate was 58.3% in group Ⅲ,less than 30% in group Ⅳ and Ⅴ.Conclusion The egg-yolk antibody against H.pylori may inhibit H.pylori infection in the stomach of BALB/c mice with IG.The protection of the antibody has a positive correlation with its dose.