Systematic analysis of T7 RNA polymerase basedin vitrolinear RNA amplification for use in microarray experiments |
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Authors: | Jörg Schneider Andreas Buneß Wolfgang Huber Joachim Volz Petra Kioschis Mathias Hafner Annemarie Poustka Holger Sültmann |
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Affiliation: | Department of Molecular Genome Analysis, German Cancer Research Centre, Heidelberg 69120, Germany. joerg.schneider@dkfz.de |
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Abstract: | Background The requirement of a large amount of high-quality RNA is a major limiting factor for microarray experiments using biopsies. An average microarray experiment requires 10–100 μg of RNA. However, due to their small size, most biopsies do not yield this amount. Several different approaches for RNA amplificationin vitrohave been described and applied for microarray studies. In most of these, systematic analyses of the potential bias introduced by the enzymatic modifications are lacking. |
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