aPlant Research International B.V., Wageningen University and Research Center, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands;bDepartment of Chemistry, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands
Abstract:
Glycoproteins from tobacco line xFxG1, in which expression of a hybrid β-(1→4)-galactosyltransferase (GalT) and a hybrid α-(1→3)-fucosyltransferase IXa (FUT9a) is combined, contained an abundance of hybrid N-glycans with Lewis X (LeX) epitopes. A comparison with N-glycan profiles from plants expressing only the hybrid β-(1→4)-galactosyltransferase suggested that the fucosylation of the LacNAc residues in line xFxG1 protected galactosylated N-glycans from endogenous plant β-galactosidase activity.