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Synthesis of Lewis X epitopes on plant N-glycans |
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| Authors: | Gerard JA Rouwendal Dion EA Florack Thamara Hesselink Jan H Cordewener Johannes PFG Helsper Dirk Bosch |
| Institution: | aPlant Research International B.V., Wageningen University and Research Center, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands;bDepartment of Chemistry, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands |
| Abstract: | Glycoproteins from tobacco line xFxG1, in which expression of a hybrid β-(1→4)-galactosyltransferase (GalT) and a hybrid α-(1→3)-fucosyltransferase IXa (FUT9a) is combined, contained an abundance of hybrid N-glycans with Lewis X (LeX) epitopes. A comparison with N-glycan profiles from plants expressing only the hybrid β-(1→4)-galactosyltransferase suggested that the fucosylation of the LacNAc residues in line xFxG1 protected galactosylated N-glycans from endogenous plant β-galactosidase activity. |
| Keywords: | Tobacco Transgenic β -(1→ 4)-Galactosyltransferase α -(1→ 3)-Fucosyltransferase N-glycan Lewis X |
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