Novel filamentous bundles in the cytoplasm of a unicellular eukaryote,Crithidia fasciculata |
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Authors: | A M Page J R Lagnado |
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Institution: | (1) Division of Biochemistry, School of Biological Sciences, Royal Holloway University of London, TW20 OEX Egham, Surrey, UK |
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Abstract: | Summary Trypanosomes, an evolutionarily ancient group of unicellular eukaryotic parasites, appear to lack both microfilaments (actin) and intermediate filaments (IFs): the major cytoskeletal component common to all trypanosomes consists of a stable microtubular array intimately associated with the plasma membrane. We present here evidence of bundles of trans-cytoplasmic filaments ca. 10 nm in diameter, seen by transmission electron microscopy, that are formed in stationary cultures of an insect trypanosome,Crithidia fasciculata. Immunofluorescent labelling with an antibody raised against plant fibrillar bundles (AFB) and Western blotting with an antibody that cross-reacts with a broad range of IFs (anti-IFA) as well as with fibrillar bundles, indicates that these filaments appear to share antigenic determinants common to animal IFs and to fibrillar bundles of plant origin.Abbreviations AFB
anti-fibrillar bundle antibody
- anti-IFA
anti-intermediate filament antibody
- IF
intermediate filament
- SEM
scanning electron microscope
- TEM
transmission electron microscope
- YOL
1/34 anti- -tubulin antibody |
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Keywords: | Trypanosome cytoskeleton Intermediate filaments Unicellular eukaryotes Immunolabelling |
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