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Isolation of potentially novel Brucella spp. from frogs
Authors:Eisenberg Tobias  Hamann Hans-Peter  Kaim Ute  Schlez Karen  Seeger Helga  Schauerte Nicole  Melzer Falk  Tomaso Herbert  Scholz Holger C  Koylass Mark S  Whatmore Adrian M  Zschöck Michael
Institution:Landesbetrieb Hessisches Landeslabor (LHL), Abteilung Veterin?rmedizin, Gie?en, Germany. tobias.eisenberg@lhl.hessen.de
Abstract:Bacterial isolates from frogs were phenotypically identified as Ochrobactrum anthropi, but 16S rRNA sequencing showed up to 100% identity with Brucella inopinata. Further analysis of recA, omp2a, omp2b, bcsp31, and IS711 and multilocus sequence analysis (MLSA) verified a close relationship with Brucella, suggesting the isolates may actually represent novel members of this growing genus of zoonotic pathogens.
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