Synthesis and properties of cationic 2'-O-[N-(4-aminobutyl)carbamoyl] modified oligonucleotides |
| |
Authors: | Seio Kohji Tokugawa Munefumi Kanamori Takashi Tsunoda Hirosuke Ohkubo Akihiro Sekine Mitsuo |
| |
Affiliation: | Department of Life Science, Tokyo Institute of Technology, J2-16, 4259 Nagatsuta, Midoriku, Yokohama 226-8501, Japan. kseio@bio.titech.ac.jp |
| |
Abstract: | 2'-O-[N-(4-Aminobutylcarbamoyl)]uridine (U(abcm)) was synthesized and incorporated into oligonucleotides. The oligonucleotides incorporating U(abcm) formed more stable duplexes with their complementary and mismatched RNAs than those containing 2'-O-carbamoyluridine (U(cm)). The stability of duplex with a U(abcm)-rG base pair showed higher thermostability than the duplex having unmodified U-rG base pair. The U(abcm) residue showed enhanced resistance to snake venome phosphodiesterase. |
| |
Keywords: | |
本文献已被 ScienceDirect PubMed 等数据库收录! |