A fungal ketoreductase domain that displays substrate-dependent stereospecificity |
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| Authors: | Zhou Hui Gao Zhizeng Qiao Kangjian Wang Jingjing Vederas John C Tang Yi |
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| Institution: | Department of Chemical and Biomolecular Engineering, University of California-Los Angeles, Los Angeles, California, USA. |
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| Abstract: | Iterative highly reducing polyketide synthases from filamentous fungi are the most complex and enigmatic type of polyketide synthase discovered to date. Here we uncover an unusual degree of programming by the hypothemycin highly reducing polyketide synthase, in which a single ketoreductase domain shows stereospecificity that is controlled by substrate length. Mapping of the structural domains responsible for this feature allowed for the biosynthesis of an unnatural diastereomer of the natural product dehydrozearalenol. |
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