Purification and some properties of a novel microbial lactate oxidase |
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Authors: | H Sztajer W Wang H Lünsdorf A Stocker R D Schmid |
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Institution: | (1) Gesellschaft für Biotechnologische Forschung, Mascheroder Weg 1, D-38124 Braunschweig, Germany. Fax: (0531) 6181 302 e-mail: HSZ@gbf-braunschweig.de , DE |
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Abstract: | Geotrichum candidum was found to produce a lactate oxidase. The enzyme was purified by gel filtration and ion-exchange chromatography. The purified
lactate oxidase showed a molecular mass of 50 kDa under denaturing and about 400 kDa under non-denaturing conditions. Transmission
electron micro-scopy analysis confirmed an octameric structure. FMN was found to be a cofactor for this enzyme. Polarographic
studies confirmed an oxygen uptake by the lactate oxidase. The enzyme showed specificity towards the L isomer of lactate and
did not oxidise pyruvate, fumarate, succinate, maleate and ascorbate. It was stable at alkaline pH and also for 15 min at
45°C. The addition of glycerol and dextran 500 000 to the enzyme sample enhanced storage stability.
Received: 28 September 1995/Received revision: 10 January 1996/Accepted: 15 January 1996 |
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