Bovine Reissner’s fiber (RF) and the central canal of the spinal cord: an immunocytochemical study using a set of monoclonal antibodies against the RF-glycoproteins |
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Authors: | J Pérez O Garrido M Cifuentes F J Alonso G Estivill-Torrús G Eller F Nualart M D López-Avalos P Fernández-Llebrez E M Rodríguez |
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Institution: | (1) Departamento de Biología Animal, Facultad de Ciencias, Universidad de Málaga, E-29071 Málaga, Spain, ES;(2) Instituto de Histología y Patología Universidad Austral de Chile, Valdivia, Chile, CL;(3) Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Málaga, Spain, ES;(4) Instituto de Immunología, Universidad Austral de Chile, Valdivia, Chile, CL |
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Abstract: | The subcommissural organ secretes N-linked complex-type glycoproteins into the cerebrospinal fluid. These glycoproteins condense to form Reissner’s fiber (RF),
which extends along the fourth ventricle and central canal of the spinal cord. A set of three monoclonal antibodies (Mabs
3E6, 3B1, and 2A5) has been obtained using these glycoproteins as immunogens. Competitive and sandwich enzyme-linked immunoassay
methods have demonstrated that the three monoclonal antibodies are directed against different epitopes, and that there is
no competition among them for their binding to glycoproteins of RF. Mab 3E6 displays immunoblotting properties that are similar
to those of a polyclonal antibody against the pool of glycoproteins from RF, but that are different from those of Mabs 3B1
and 2A5. All three antibodies immunostain the bovine subcommissural organ and RF. A population of ependymal cells is stained
by the polyclonal antibody, and Mabs 2A5 and 3E6, but not by Mab 3B1. The material present in a population of ependymal cells
of the central canal, and the glycoproteins secreted by the subcommissural organ thus probably have partial chemical identity.
Some evidence suggests that the immunoreactive ependymal cells are secretory cells. The luminal surface of the central canal
is coated by a thin layer of material with immunocytochemical characteristics different from those of the ependymal cells;
such a coat may correspond to material released from RF.
Received: 19 December 1995 / Accepted: 30 April 1996 |
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Keywords: | : Subcommissural organ Reissner’ s fiber Central canal Monoclonal antibodies Bovine |
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