A transient duplication of the acetolactate synthase gene in a cell culture of Datura innoxia |
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Authors: | W. Xiao P. K. Saxena J. King G. H. Rank |
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Affiliation: | (1) Department of Biology, University of Saskatchewan, S7N 0W0 Saskatoon, Saskatchewan, Canada |
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Abstract: | Summary A 2.0 kb fragment of the yeast ILV2 gene, which codes for the target enzyme acetolactate synthase (ALS) of the herbicide chlorsulfuron, was shown to hybridize to the nuclear DNA of a haploid cell culture of Datura innoxia P. Mill. Nuclear DNA of a chlorsulfuron resistant line of D. innoxia, CSR6, gave a prominent 2.65 kb band when cleaved by either EcoRI or HindIII. The 2.65 kb band has been shown to hybridize with the yeast ILV2 probe. A herbicide resistant line descended from CSR6 by continuous culture resulted in the loss of the 2.65 kb restriction fragment. These observations suggest that CSR6 resulted from a large tandem duplication of the ALS gene and that a point mutation for herbicide resistance in an ALS gene repeat unit of the duplication was selected during subsequent growth of the resistant line. |
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Keywords: | Gene amplification Datura Sulfonylurea resistance Plant cell culture |
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