| 用生物素标记DNA探针进行菌落原位杂交 |
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| 引用本文： | 蒋三亮,张思仲.用生物素标记DNA探针进行菌落原位杂交[J].微生物学通报,1992,19(3):168-170. |
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| 作者姓名： | 蒋三亮 张思仲 |
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| 作者单位： | 华西医科大学医学遗传研究室，华西医科大学医学遗传研究室 四川成都 610041，四川成都 610041 |
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| 基金项目： | 国家自然科学基金，计划生育委员会基金 |
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| 摘 要： |
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| 关 键 词： | 菌落原位杂交 生物素标记 DNA探针 |
COLONY HYBRIDIZATION WITH BIOTIN LABELED DNA PROBE |
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| Jiang Sanliang Zhang Sizhong.COLONY HYBRIDIZATION WITH BIOTIN LABELED DNA PROBE[J].Microbiology,1992,19(3):168-170. |
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| Authors: | Jiang Sanliang Zhang Sizhong |
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| Abstract: | A simple, rapid method for colony hybridization has been developed. The DNA probes were labeled by digo xigenin. The signal of hybridization was detected by streptavidin and poly (AP) system. The results showed that this method is sensitive, specific and repoducible, it can be used for colony hybridization instead of isotopic. |
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| Keywords: | Biotin labeled DNA probe Colony hybridization Colony screening |
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