Immunohistochemical studies with antibodies to myosins from the cytoplasm and membrane fraction of human blood platelets |
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| Authors: | Prof. Dr. Ute Gröschel-Stewart Christine Rakousky Renate Franke Itzhak Peleg Itkhak Kahane Amiram Eldor Andras Muhlrad |
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| Affiliation: | (1) Institut für Zoologie, Technische Hochschule Darmstadt, Bundesrepublik Deutschland;(2) The Hebrew University Hadassah Medical Center, Jerusalem, Israel;(3) Institut für Zoologie, Schnittspahnstrasse 10, 6100 Darmstadt, Germany |
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| Abstract: | Summary Antibodies were raised to myosins extracted from the cytoplasm and solubilized membranes of human blood platelets. Both antibodies had similar titers as shown by enzyme-immunoassay and bound to the same sites as shown by immunohistochemistry. They were specific for cytoplasmic myosins (e.g., in human white blood cells, platelets and fibroblasts and rat endothelial cells). They showed no crossreaction with human or rat smooth muscle.Abbreviations ATPase adenosine triphosphatase EC 3.6.1.3 - FITC fluorescein isothiocyanate |
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| Keywords: | Platelet membrane Platelet cytoplasm Myosin Enzyme immunoassay Immunofluorescence Man |
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