WLIP, a lipodepsipeptide of Pseudomonas 'reactans', as inhibitor of the symptoms of the brown blotch disease of Agaricus bisporus |
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Authors: | C Soler-Rivas N Arpin J M Olivier H J Wichers |
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Institution: | Agrotechnological Research Institute (ATO-DLO), Wageningen, The Netherlands,;Laboratoire de Mycochimie, Institut de Chimie et de Biologie moleculaire et cellulaire, UniversitéClaude Bernard, Lyon, and;Institut National de la Recherche Agronomique, Station de Recherches sur les Champignons, Centre de Recherches de Bordeaux, France |
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Abstract: | A cell-free crude extract containing the white line inducing principle (WLIP), a lipodepsipeptide produced by Pseudomonas 'reactans' , could inhibit browning of mushrooms caused by Pseudomonas tolaasii . Mushrooms inoculated with Ps. tolaasii at concentrations of 2·7 × 106 cfu ml−1 or higher showed the symptoms of the disease after 2 d of incubation. Mushroom caps treated with various concentrations of a crude WLIP preparation, and later inoculated with bacterial concentrations higher than the threshold value, did not develop the symptoms of the disease. One milligram of a crude WLIP preparation could block 50% of the symptoms caused by 1·2 × 107 cfu. The inhibition of browning was effective when incubating at low temperatures for 4 d. A suspension containing 1·6 mg ml−1 of pure WLIP was also able to inhibit the symptoms of brown blotch disease induced by 7·6 × 106 cfu ml−1 of Ps. tolaasii . |
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