Expression of human 5-lipoxygenase cDNA in Escherichia coli |
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Authors: | M Noguchi T Matsumoto M Nakamura M Noma |
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Affiliation: | Life Science Research Laboratory, Japan Tobacco Inc., Yokohama. |
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Abstract: | The cis/trans interconversion of Glt-Ala-Ala-Pro-Phe-4-nitroanilide and Glt-Ala-Gly-Pro-Phe-4-nitroanilide was studied both enzymatically and nonenzymatically by measuring kinetic β-deuterium isotope effects. The hydrogen atom at the -carbon atom of the Xaa residue within the Xaa-Pro moiety was substituted by deuterium. In the nonenzymatic case the transition state of rotation is reflected by kH/kD > 1. When catalysed by 17 kDa PPIase the same bond rotation is characterized by kH/kD < 1. This suggests a covalent mechanism of catalysis which involves an approximately tetravalent carbon of the prolyl imidic bond for the transition state of reaction. |
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Keywords: | cis/trans interconversion Prolyl imidic bond Deuterium isotope effect Prolyl cis/trans-isomerase Cyclophilin Cyclosporin |
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