中国10个民族永生细胞系的建立与保存

永久保存我国各少数民族的遗传信息是中国人类基因组计划的重要内容，为此，采用EB病毒(epstein-barr Svirus EBV)上清液及Hepes转化外周血B淋巴细胞，并加入环孢霉素A(cyclosporine A)抑制T淋巴细胞，成功地对中国哈萨克族、满族、朝鲜族、赫哲族、蒙古族、锡伯族、回族、布依族、四川汉族和福建汉族的部分个体建立了永生细胞系。其中哈萨克族64株，朝鲜族58株，赫哲族18株，锡伯族43株，回族63株，布依族67株，满族65株，蒙古族62株，四川汉族51株，福建汉族58株。总计549株。为保存我国各民族遗传资源、分析各少数民族间的遗传学差异及其起源，奠定了材料基础。 Establishment and Preservation of Immortal Lymphoblastoid Cell Lines of the 10 Ethnic Groups in China HUANG Xiao-yi,LIU An,YU Yang,MA Lin-lin,SHI Rong-qian,Lü Fu-qu,JIANG Yan,SUN Wen-jing,XUE Ya-li,FU Song-bin,LI Pu Department of Medical Genetics,Harbin Medical University,Harbin 150086,China Abstract:The immortal lymphoblastoid cell lines were established by EBV transformation of B cells and addition of cyclosporin A to inhibit the activity of T cells.In the present study,549 immortal cell lines of different ethnic groups of Hazak,Manchu,Korea,Hozhe,Mongolia,Sibe,Hui, Puyi,Han in Fujian and Han in Sichuan were established.Through our research,we found it is harmful for B lymphocytes to transform if excessful leucocytes are inoculated.And it is crisis that cyclosporine A shoud be added the last.Our work is an important part of the research of human genome diversity for the exploration of the origin and evolution of different ethnic groups,and it also provides enough research materials for further studies.Moreover,we have sent 50 cell lines of Hozhe,Mongolia,Sibe,Daur,Oroqen to CEPH.Thus it is possible for us to utilize the genetic resources of CEPH freely. Key words：immortal lymphoblastoid cell line；EBV；ethnic groups；reserved in liquid-nitrogen

Establishment and Preservation of Immortal Lymphoblastoid Cell Lines of the 10 Ethnic Groups in China

The immortal lymphoblastoid cell lines were established by EBV transformation of B cells and addition of cyclosporin A to inhibit the activity of T cells.In the present study,549 immortal cell lines of different ethnic groups of Hazak, Manchu, Korea, Hozhe, Mongolia, Sibe, Hui, Puyi, Han in Fujian and Han in Sichuan were established.Through our research,we found it is harmful for B lymphocytes to transform if excessful leucocytes are inoculated.And it is crisis that cyclosporine A shoud be added the last.Our work is an important part of the research of human genome diversity for the exploration of the origin and evolution of different ethnic groups,and it also provides enough research materials for further studies.Moreover,we have sent 50 cell lines of Hozhe, Mongolia, Sibe, Daur, Oroqen to CEPH.Thus it is possible for us to utilize the genetic resources of CEPH freely.